Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome. By Jay Shendure, Gregory J. Porreca, Nikos B. Reppas, Xiaoxia Lin. Request PDF on ResearchGate | Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome | We describe a DNA sequencing technology in which . We apply this technology to resequence an evolved strain of Escherichia coli at less Accurate multiplex polony sequencing of an evolved bacterial genome.
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McCutcheon and Abraham M. This paper has highly influenced 23 other papers.
Church Published in Science We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. References Publications referenced by this paper.
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This entry form currently does not support special characters. Accurate multiplex polony sequencing of an evolved bacterial genome. Mjltiplex does not store recipient email addresses. We apply this technology to resequence an evolved strain of Escherichia coli at less than one error per million consensus bases.
Accurate multiplex polony sequencing of an evolved bacterial genome.
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We describe a DNA sequencing technology in which a commonly available, inexpensive epifluorescence microscope is converted to rapid nonelectrophoretic DNA sequencing automation. Overview of DNA sequencing strategies.
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This paper has been referenced on Twitter 9 times over the past 90 days. A cell-free, mate-paired library provided single DNA molecules that were amplified in parallel to 1-micrometer beads by emulsion polymerase chain reaction. A cell-free, mate-paired library provided single DNA molecules that were amplified in parallel to 1-micrometer beads by emulsion polymerase chain reaction.
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Accurate Multiplex Polony Sequencing of an Evolved Bacterial Genome
From This Paper Figures, tables, and topics from this paper. Millions of beads were seqeuncing in a polyacrylamide gel and subjected to automated cycles of sequencing by ligation and four-color imaging. I am a Faculty Member who recommended this article.
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Our protocols were implemented with off-the-shelf instrumentation and reagents.
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You have a close personal relationship e. Base polyacrylamide gels Ligation. Sequencinh per base was roughly one-ninth as much as that of conventional sequencing.
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